eskape bacteria  (ATCC)

Journal: Frontiers in Microbiology

Article Title: Restriction of growth and biofilm formation of ESKAPE pathogens by caprine gut-derived probiotic bacteria

doi: 10.3389/fmicb.2024.1428808

Figure Lengend Snippet: Zone of inhibition of ESKAPE pathogen growth by cell-free supernatant and cell-lysates of LAB isolates. The figure depicts the zone of inhibition radius from agar well diffusion assay. The zone of inhibition was recorded with the zone of inhibition scale (PW297, Himedia) following 24 h of incubation after the addition of sterile-filtered (A) cell-free culture supernatants, and (B) cell lysate of Lactobacillus isolates to the agar-wells. The horizontal bar depicts the Mean (values embedded) of the zone of inhibition radius of three experiments (mm) excluding the diameter of the agar well; Lp. plantarum (MTCC-2621) used as a positive control.

Article Snippet: Pathogenic ESKAPE group bacteria were obtained from: E. coli and S. aureus (American Type Culture collection-ATCC, United States), K. pneumonae , A. baumanii , P. aeruginosa (MTCC, Chandigarh, India), and E. faecalis (National Center for Microbial Resources-NCMR, Pune, India).

Techniques: Inhibition, Diffusion-based Assay, Incubation, Sterility, Positive Control

Journal: Frontiers in Microbiology

Article Title: Restriction of growth and biofilm formation of ESKAPE pathogens by caprine gut-derived probiotic bacteria

doi: 10.3389/fmicb.2024.1428808

Figure Lengend Snippet: Lactobacillus isolates prevent biofilm formation by ESKAPE pathogens. The biofilm inhibition ability of Lactobacillus isolates was assessed using Cell-free culture filtrate (CSF). The assay was performed in 24-well polystyrene plates. ESKAPE pathogens [ (A) E. faecalis, (B) S. aureus, (C) K. pneumoniae, (D) A. baumannii, (E) P. aeruginosa, and (F) E. coli] were cultured either in the presence or absence of Lactobacillus-CSF and incubated in static conditions at 37 ˚C for 48 hours. Subsequently, quantitative measurement of biofilm was performed using the standard crystal violet-based assay. The bar diagrams depict the Mean ± SD of the absorbance (A 570nm ) values from triplicate wells. The experiment was performed twice. One-way ANOVA was performed to compare the mean differences of each condition compared to the base value without any CSF (negative control biofilm, CB). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. All Lactobacillus -CSF exhibited various degrees of biofilm inhibition on ESKAPE bacteria. Lp plantarum (MTCC-2621) used as a positive control.

Article Snippet: Pathogenic ESKAPE group bacteria were obtained from: E. coli and S. aureus (American Type Culture collection-ATCC, United States), K. pneumonae , A. baumanii , P. aeruginosa (MTCC, Chandigarh, India), and E. faecalis (National Center for Microbial Resources-NCMR, Pune, India).

Techniques: Inhibition, Cell Culture, Incubation, Crystal Violet Assay, Negative Control, Bacteria, Positive Control